AMSBIO has published a comprehensive online article highlighting the critical role of culture techniques in the use of induced pluripotent stem cells (iPSCs) for drug discovery and therapeutic applications. iPSCs represent an invaluable tool in these areas, but their effectiveness depends on the ability to generate homogeneous cultures.
The article highlights the importance of maintaining genetic stability, optimizing culture conditions, and effectively managing single-cell expansion to ensure reproducibility and product quality. It discusses the use of chemically defined media such as the advanced product StemFit®, which provides precise control over the cell growth environment, thus ensuring consistency across experiments. This consistency is essential for developing scalable protocols for iPSC culture and differentiation, which are critical for clinical application.
Furthermore, the article discusses the important role of cryopreservation in the preservation of iPSCs and organoids, emphasizing the importance of using chemically defined cryopreservation media of animal origin such as STEM-CELLBANKER® to achieve high yields and ensure long-term viability of the stem cells even after prolonged storage.
Looking ahead, the article examines challenges in further developing 3D iPSC cultures, such as uncontrolled spheroid aggregation and cell shear stress, and presents a novel approach using laminin E8 fragments to generate an effective extracellular matrix (ECM) that promotes efficient stem cell production.
Read the full article.
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